常用荧光染料的激发波长和发射波长:
荧光染料 Fluorescent Dye |
激发波长(nm) Excitation |
发射波长(nm) Emission |
Cy2 |
489 |
506 |
GFP(Red Shifted) |
488 |
507 |
YO-PRO-1 |
491 |
509 |
YOYO-1 |
491 |
509 |
Calcein |
494 |
517 |
FITC |
494 |
518 |
FluorX |
494 |
519 |
Alexa 488 |
490 |
520 |
Rhodamine 110 |
496 |
520 |
ABI,5-FAM |
494 |
522 |
Oregon Green 500 |
503 |
522 |
Oregon Green 488 |
496 |
524 |
Rlbo Green |
500 |
525 |
Rhodamine Green |
502 |
527 |
Rhodamine 123 |
507 |
529 |
Magnesium Green |
506 |
531 |
Calcium Green |
506 |
533 |
TO-PRO-1 |
514 |
533 |
TOTO-1 |
514 |
533 |
ABI,JOE |
520 |
548 |
BODIPY 530/550 |
530 |
550 |
Dil |
549 |
565 |
BODIPY R |
542 |
568 |
BODIPY 558/568 |
558 |
568 |
BODIPY 564/570 |
564 |
570 |
Cy3 |
550 |
570 |
Alexa 546 |
555 |
570 |
TRITC |
547 |
572 |
Magnesium Orange |
550 |
575 |
Phycoerythrin, R&B |
565 |
575 |
Rhodamine Phalloidin |
550 |
575 |
Calcium Orange |
549 |
576 |
Pyronin Y |
555 |
580 |
Rhodamine B |
555 |
580 |
ABI,TAMRA |
560 |
582 |
Rhodamine Red |
570 |
590 |
Cy3.5 |
581 |
596 |
ABI,ROX |
588 |
608 |
Calcium Crimson |
590 |
615 |
Alexa 594 |
590 |
615 |
Texas Red |
595 |
615 |
Nile Red |
549 |
628 |
YO-PRO-3 |
612 |
631 |
YOYO-3 |
612 |
631 |
R-Phycocyanin |
618 |
642 |
C-Phycocyanin |
620 |
648 |
TO-PRO-3 |
642 |
660 |
TOTO-3 |
642 |
660 |
DiD DilC’(5) |
644 |
665 |
Cy5 |
649 |
670 |
Thiadicarbocyanine |
651 |
671 |
Cy5.5 |
675 |
694 |