Adjust the contrast if neceesary: Image/Adjust/BrightnessConstrast
Change or check all Images are in 8-bit format. Image/Type/8-bit
Image/Color/Merge Channels and the Merge Channels box will appear
Select the fluorescent images in the appropriate R, G and B channels and the DIC or similar image in gray channel. Select “Create Composite” and click “OK”
Click on the new ‘composite’ image to activate it
Image/Type/RGB Color
Save it as “Tiff” file
posted on
2017-07-16 05:34小马嘚儿嘚儿
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