dockerfile 示例

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FROM **** RUN  dnf install -y R  openssl-devel && \      R -e "install.packages(c('getopt','BiocManager'), repos='https://cran.r-project.org/')" && \      R -e "BiocManager::install('org.Mm.eg.db')" && \      R -e "BiocManager::install('org.Hs.eg.db')" && \      R -e "BiocManager::install('clusterProfiler')" && \      R -e "remove.packages('rvcheck')" && \      R -e "install.packages('https://cran.r-project.org/src/contrib/Archive/rvcheck/rvcheck_0.1.8.tar.gz', repos=NULL, type='source')" && \      R -e "BiocManager::install('clusterProfiler')"   RUN dnf install -y libcurl-devel R-rgeos && \     R -e "install.packages(c('httr','plotly','SeuratObject','Seurat','tidyverse','extrafont','ggplot2','data.table'),repos='https://cran.r-project.org/')"  && \     R -e "BiocManager::install('monocle')" && \     R -e "install.packages('pheatmap', repos='https://cran.r-project.org/')"   COPY ./tools/  /usr/lib/ RUN pip install  cutadapt && \     dnf install -y  java-1.8.0-openjdk.x86_64 perl htslib samtools  && \     chmod a+x /usr/lib/FastQC/fastqc && \     ln -s /usr/lib/FastQC/fastqc  /usr/local/bin/fastqc && \     ln -s /usr/lib/TrimGalore-0.6.6/trim_galore /usr/local/bin/trim_galore && \     ln -s /usr/lib/hisat2-2.2.1/hisat2 /usr/local/bin/hisat2 && \     ln -s /usr/lib/hisat2-2.2.1/hisat2-build /usr/local/bin/hisat2-build && \     ln -s /usr/lib/stringtie-2.2.1.Linux_x86_64/stringtie  /usr/local/bin/stringtie && \     ln -s /usr/lib/stringtie-2.2.1.Linux_x86_64/prepDE.py3  /usr/local/bin/prepDE.py3 && \     ln -s /usr/lib/STAR-2.7.10a/STAR  /usr/local/bin/STAR  && \     export PATH=$PATH:/usr/lib/sratoolkit.3.0.0 RUN R -e "install.packages(c('gplots','RColorBrewer'),repos='https://cran.r-project.org/')" && \     R -e "install.packages('https://cran.r-project.org/src/contrib/Archive/locfit/locfit_1.5-9.2.tar.gz', repos=NULL)" && \     R -e "BiocManager::install('DESeq2')"   RUN R -e "BiocManager::install('org.Mm.eg.db')"  && \     R -e "BiocManager::install('MAST')"  && \     R -e "BiocManager::install('optparse')"