centos7 中安装 busco

 

001、系统

[root@PC1 software]# cat /etc/redhat-release
CentOS Linux release 7.9.2009 (Core)

 

002、python版本

[root@PC1 software]# python3 --version
Python 3.11.3

 

003、gcc版本

[root@PC1 software]# gcc --version
gcc (GCC) 4.8.5 20150623 (Red Hat 4.8.5-44)
Copyright (C) 2015 Free Software Foundation, Inc.
This is free software; see the source for copying conditions.  There is NO
warranty; not even for MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.

 

003、git下载

[root@PC1 software]# git clone https://gitlab.com/ezlab/busco.git

 

004、执行安装程序

[root@PC1 software]# cd busco/
[root@PC1 busco]# ls
bin  CHANGELOG  config  CONTRIBUTING.md  LICENSE  README.md  scripts  setup.py  src  test_data  tests
[root@PC1 busco]# python3 setup.py install --user

 

005、测试程序

[root@PC1 busco]# cd bin/
[root@PC1 bin]# ls
busco
[root@PC1 bin]# ./busco --help | head
usage: busco -i [SEQUENCE_FILE] -l [LINEAGE] -o [OUTPUT_NAME] -m [MODE] [OTHER OPTIONS]

Welcome to BUSCO 5.4.7: the Benchmarking Universal Single-Copy Ortholog assessment tool.
For more detailed usage information, please review the README file provided with this distribution and the BUSCO user guide. Visit this page https://gitlab.com/ezlab/busco#how-to-cite-busco to see how to cite BUSCO

optional arguments:
  -i SEQUENCE_FILE, --in SEQUENCE_FILE
                        Input sequence file in FASTA format. Can be an assembled genome or transcriptome (DNA), or protein sequences from an annotated gene set. Also possible to use a path to a directory containing multiple input files.
  -o OUTPUT, --out OUTPUT
                        Give your analysis run a recognisable short name. Output folders and files will be labelled with this name. The path to the output folder is set with --out_path.