stringtie 软件的安装

 

001、conda直接安装

conda install -c bioconda stringtie

 

002、调用测试

root@DESKTOP-A31BQ38:~/anaconda3/bin# ./stringtie --help | head
StringTie v2.1.7 usage:

stringtie <in.bam ..> [-G <guide_gff>] [-l <prefix>] [-o <out.gtf>] [-p <cpus>]
 [-v] [-a <min_anchor_len>] [-m <min_len>] [-j <min_anchor_cov>] [-f <min_iso>]
 [-c <min_bundle_cov>] [-g <bdist>] [-u] [-L] [-e] [--viral] [-E <err_margin>]
 [--ptf <f_tab>] [-x <seqid,..>] [-A <gene_abund.out>] [-h] {-B|-b <dir_path>}
 [--mix] [--conservative] [--rf] [--fr]
Assemble RNA-Seq alignments into potential transcripts.
Options:
 --version : print just the version at stdout and exit