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A high min_kmer value was used to reduce noise in the assembly and to identify only transcripts that were relatively highly expressed. At lower min_km... 阅读全文
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Centering: 使数据的均值（Empirical Mean）变为0的过程。 具体操作：原始数据Xn(n=1, 2, 3, ..., n)减去均值. Scaling: 使数据的标准差（Empirical Standard Deviation）变为1的过程。 具体操作：原始数据Xn(n=1,... 阅读全文
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```
curl https://www.msi.umn.edu/tutorial-materials >tmp.txt
perl -alne ‘{/(https.*?pdf)/;print $1 if $1}’ tmp.txt >pdf.address
perl -alne ‘{/(https.*?txt)/;print $1 if $1}’ tmp.txt
perl -alne ‘{/(htt... 阅读全文
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```
重复序列的种类 Tandem repeats 串连重复 Satellite DNA 卫星DNA Variable number tandem repeat /Minisatellite 小卫星 Short tandem repeat（STR）/Microsatellite (Trinucleotide repeat disorders)微卫星 Interspersed... 阅读全文
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``` x<-seq(-1.1,1.1,length = 3000) rep<-30 y<-matrix(,3000,rep) yx<-matrix(,3000,rep) xx<-matrix(,3000,rep) c<-seq(0,1,length=rep) for (i in 1:rep) for (p in 1:3000){ y[p,i]<-(1-(x[p])^2)^(1/2)+((x[p]... 阅读全文
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http://www.sciencedirect.com/science/journal/10557903/81 阅读全文
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```#!/usr/bin/env python# usages: python extract_seq_by_pos.py input.fasta id_start_end > result.fastaimport sysimport reFASTA= sys.argv[1]BED= sys.ar... 阅读全文
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```sudo sugpg --keyserver keyserver.ubuntu.com --recv-keys 51716619E084DAB9gpg -a --export 51716619E084DAB9 |apt-key add -``` 阅读全文
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How to fix the sources listSometimes the apt-get may not work, it is often caused by the misspelled sources list. In most cases, you can fix it by you... 阅读全文
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```java -jar /home/liuhui/bin/trinityrnaseq_r20140413p1/trinity-plugins/Trimmomatic/trimmomatic.jar PE -threads 20 -phred33 P_ACAGTG_L002_R1_001.fastq... 阅读全文