blast 和 blast+ 的一点区别 -- 以 tblastn 为例
待比对序列:包括 stop codon 在内,总长 46 aa。
>PITAhm_001998-RA not_annotated
MEVSIKTLGFFSSGQISMTLAQKDLTVRGFTYAPGTHYSILQIFW*
其在基因组上的注释:总长 138 nt。
# gff
scaffold511108 merged mRNA 110192 110329 . + . ID=PITAhm_001998-RA;Parent=PITAhm_001998;Name=PITAhm_001998-RA;Alias=non-annotated model,no protein domains,note:complete
scaffold511108 merged exon 110192 110329 100 + . ID=PITAhm_001998-RA:1;Parent=PITAhm_001998-RA
scaffold511108 merged CDS 110192 110329 100 + 0 ID=PITAhm_001998-RA:cds;Parent=PITAhm_001998-RA
blast 比对结果:
# tblastn (blastall)
PITAhm_001998-RA scaffold511108 100.00 45 0 0 1 45 110192 110326 8e-18 94.0
# tblastn (blast+)
PITAhm_001998-RA scaffold511108 100.00 46 0 0 1 46 110192 110329 6e-21 94.4