PacBio软件总览 - 初级分析

PacBio® RS Software Overview

• PacBio运行的整个流程是什么？每一步都用到了什么软件？

PacBio软件套件

RS Remote：Design runs remotely，Can assign multiple SMRT® Cells per well with different movie times

RS Touch：Loading a Run，Monitor at the instrument or remotely，View real-time base incorporations，Displays remaining run time，Shows SMRT® Cell status, from cell prep to Quality Values

PacBio® RS：测序仪

Primary Analysis Pipeline：

SMRT® Analysis – Secondary Analysis：

SMRT Portal：Job Selection（Prior jobs are searchable，Can select one or more jobs），Report Viewing（）

SMRT View：Genome Browser，View Base Modification Details

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Primary Analysis

• PacBio测序仪的光学原理，如何得到的fastq文件
• 测序结果都有哪些文件，各代表什么意思？

PacBio的光学检测系统：

Algorithms reduce photon counts vs. time to sequences in real time