单细胞 | Entropy

 

We quantified this time dependent increase in basal-luminal mixing using entropy of cell-type probabilities, which robustly agrees with our per-sample measures of plasticity while capturing a range of plasticity within each time point and condition

 

Cellular plasticity increases as a function of JAK/STAT signaling. Plasticity is measured as the average entropy of cell-type classification probabilities per metacell. JAK/STAT score is the average Z-score of the leading-edge gene subset of KEGG_JAK_STAT_SIGNALING_PATHWAY and HALLMARK_ IL6_JAK_STAT3_SIGNALING. The entropy of classification probabilities was first calculated for each single cell and then averaged per meta cell for visualization. A linear fit is shown with a Pearson’s correlation of 0.76.

 

projects/ApcKO_multiomics/lineagePlasticity/SCENT/SCENT.ipynb

 

 

参考：

• 单细胞RNA-seq分析：基于单细胞熵确定细胞分化及谱系
• 用单细胞熵(SCENT package)估计单细胞分化潜能