单细胞数据normalization方法 | SCTransform

SCTransform

Normalization and variance stabilization of single-cell RNA-seq data using regularized negative binomial regression

ChristophH/sctransform

 

Variance Stabilizing Transformation 

 

 

It is obvious that the variance of counts is generally greater than their mean, especially for genes expressed at a higher level.

There are two mathematically equivalent formulations of the NB distribution.

In its traditional form, which I will mention only for the sake of completion, the NB distribution estimates the probability of having a number of failures until a specified number of successes occur. 

The second definition sounds more intimidating but is much more useful. The NB distribution can be defined as a Poisson-Gamma mixture distribution.