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I've been running BizTalk 2009 on my Windows 2008 SP2 X86 machine, and after installing .NET Framework 4.0, the Enterprise Single Sign-On Service stopped working.

It failed with the following error:

Could not create SSOSQL. To fix the problem, reinstall SSO or try 'regasm SSOSQL.dll' from a Visual Studio command prompt.
Error Code: 0x80131700

The file is located at C:\Program Files\Common Files\Enterprise Single Sign-On\SSOSQL.dll

Run Visual Studio Commmand prompt as Administrator and run 'regasm "C:\Program Files\Common Files\Enterprise Single Sign-On\SSOSQL.dll"'.