摘要： getopts的使用问题 #!/usr/bin/perl #execute ABC.pl -s 999 -g -t use Getopt::Std; getopts('s:t:g:',\%opts); foreach my $key ( keys %opts){ print $key,"-->;", 阅读全文

摘要： megablast 采用贪婪式算法，速度较一般blast快，多用于数据量大且序列相似性较高的情况。megablast 参数说明./megablast --helpmegablast 2.2.11 arguments: -d Database [String] default = nr -i Query File [File In] -e Expectation value [Real] default = 10.0 -m alignment view options: 0 = pairwise, 1 = query-anchored showing i... 阅读全文

摘要： formatdb is an outdated software tool in molecular bioinformatics to format protein or nucleotide databases for BLAST. It has been replaced by the tool makeblastdb and the NCBI "strongly encourage[s]" users to stop using formatdb.The formatdb.exe program is part of the BLAST release, which 阅读全文

摘要： 根据oracle文档的解释： Cause: The control file change sequence number in the datafile is greater than the number in the control file. This implies that the wrong control file is being used. Note that repea... 阅读全文

摘要： 命令行执行：ppm install padwalker-------------------------------------------------------------C:\Documents and Settings\Administrator>ppm install padwalkerDownloading ActiveState Package Repository packlist...doneUpdating ActiveState Package Repository database...doneSyncing site PPM database with .pac 阅读全文

摘要： 现象： Eclipse IDE for Java Developers Version: Helios Service Release 1，已经安装了epic和padwalker。perl环境装的是activePerl。没有手动设置过系统的参数和Eclipse的参数。 可以正常Run，但是不能Deb 阅读全文

摘要： The Burrows–Wheeler transform (BWT, also called block-sorting compression), is an algorithm used in data compression techniques such as bzip2. It was invented by Michael Burrows and David Wheeler in 1... 阅读全文

摘要： What is Bowtie 2? Bowtie 2 is an ultrafast and memory-efficient tool for aligning sequencing reads to long reference sequences. It is particularly good at aligning reads of about 50 up to 100s or 1,0... 阅读全文

摘要： 解读报告前需要掌握的概念 alignments 代表比对上的两个序列 hits 表示两个序列比对上的片段 Score 比对得分，如果序列匹配上得分，不一样，减分，分值越高，两个序列相似性越高 E Value 值越小，越可信，相对的一个统计值。 Length 输入序列的长度 Identities 一致性，就是两个序列有多少是一样的 Query 代表输入序列... 阅读全文

摘要： 安装Randfold 软件下载：http://bioinformatics.psb.ugent.be/supplementary_data/erbon/nov2003/ 安装：tar zxvf randfold.tar.gz cd randfold-2.0 make cp randfold /usr/local/bin ... 阅读全文