摘要： From:http://www.cureffi.org/2012/12/19/forward-and-reverse-reads-in-paired-end-sequencing/Illustration for mate pair sequencing：http://www.investigativegenetics.com/content/2/1/23/figure/F2?highres=yhttp://seqanswers.com/forums/showthread.php?t=15626DNA 复制：只能从5'端向3'端（相对引物链，即待构造的链来说）延长。 ---&g 阅读全文