摘要: Transcriptome assembly and differential expression analysis for RNA-Seq.Cufflinks assembles transcripts, estimates their abundances, and tests for dif... 阅读全文
posted @ 2016-01-20 17:55 SamYangBio 阅读(377) 评论(0) 推荐(0) 编辑
摘要: What is TopHat?TopHat is a program that aligns RNA-Seq reads to a genome in order to identify exon-exon splice junctions. It is built on the ultrafast... 阅读全文
posted @ 2016-01-20 17:46 SamYangBio 阅读(464) 评论(0) 推荐(0) 编辑
摘要: 1.对于长度大于50bp的reads,bowtie2更精确;而小于50bp的reads,bowtie1更精确更快速2.bowtie2支持的reads长度没有上限,当然reads长度在50~1000bp为宜;而bowtie1支持reads长度最长约为1000bp3.bowtie2的对比支持gap,而b... 阅读全文
posted @ 2016-01-20 17:08 SamYangBio 阅读(1153) 评论(0) 推荐(0) 编辑