解决samtools报错:[main_samview] region "chr2:20,100,000-20,200,000" specifies an unknown reference name. Continue anyway.

根据Samtool 的manual文档介绍,如果想搜索bam文件的某段区域,需要用到以下命令:

samtools view aln.sorted.bam chr2:20,100,000-20,200,000

  于是聪明的我也跟着命令搜索了这段区域的序列,结果报错:[main_samview] region "chr2:20,100,000-20,200,000" specifies an unknown reference name. Continue anyway.

搜索了一堆资料后,直到遇见了这个问题,里面有一句话是这么说的:there is no such chromosome "chr2" in the bam. try "2". 瞬间开悟,于是将命令行的chr2修改为2,见如下命令:

samtools view aln.sorted.bam 2:20,100,000-20,200,000

  成功解决了

 

参考链接:

https://www.biostars.org/p/251354/

http://www.htslib.org/doc/samtools.html

posted @ 2018-06-15 17:17  橙子牛奶糖  阅读(1781)  评论(0编辑  收藏  举报