摘要: 1. Trinity进行转录组组装Trinity进行转录组组装的典型命令如下:$ /opt/biosoft/trinityrnaseq_r20131110/Trinity.pl --seqType fq --JM 50G\ --left sample1_1.clean.fastq sample2_1.clean.fastq\ --right sample1_2.clean.fastq sample2_2.clean.fastq\ --jaccard_clip --CPU 6 --SS_lib_type FR–JM后的参数设定与转录组的大小有关,在内存足够的情况下,设定大点能节约时间;–left 阅读全文
posted @ 2014-03-18 15:52 沉下心学习 阅读(6433) 评论(1) 推荐(0) 编辑
摘要: 原文:http://www.biostars.org/p/53528/This tutorial also serves as the supporting information for Lecture 9 of the course titledAnalyzing High Througput Sequencing Dataoffered at Penn State.Within this tutorial we compare the efficiency and characteristics of several software tools designed to filter F 阅读全文
posted @ 2014-03-18 08:50 沉下心学习 阅读(3636) 评论(0) 推荐(0) 编辑